Streptomyces sp. M-20 균의 대사물에 의한 Streptococcus mutas의 Glucosyltransferase 활성 억제 효과

• 영문명: Inhibitory Effect of Metabolites isolated from Streptococcus mutas sp. M-20 on Glucosyltransferase Activity from Streptococcus mutas
• 발행기관: 대한약학회
• 저자명: 김경자(Kyoung-Ja Kim)
• 간행물 정보: 『약학회지』제49권 제2호 (2005년), 122~127쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2005.04.30

영문 초록

Dental caries is one of the most common oral diseases in the world. Glucosyltransferase (Gtase) of Streptococcus mutans (S. mutans) plays an important role in the development of dental caries. For the purpose to develop anti- caries, we examined the effect of metabolites isolated from Streptomyces sp. M-20 on Gtase and the growth of S. mutans. Streptomyces sp. M-20 isolated from Mongolian soil showed 95~96% sequence homology with that of Streptomyces lin- colnensis. The metabolites of Streptomyces sp. M-20 were partially purified by extraction with ethyl acetate, silica gel column chromatography and preparative TLC. Partially purified metabolite, red colored component (MR-20) in ethyl acetate fraction showed potent antibacterial activitiy against S. mutans and inhibitory activity against Gtase purified from S. mutans, while another isolated yellow component (MY-20) showed no activity against S. mutans. The inhibitory activity of MR-20 against Gtase was confirmed by activity staining on SDS-polyacrylamide gel electrophoresis. The concentration of MR-20 for 50% inhibition (IC50) against Gtase activity was 60㎍/㎖. These results suggest that MR-20 can be developed for antibacterial agent and anticaries.

키워드

glucosyltransferase(GTase) Streptomyces sp. M-20 Streptococcus mutans anticaries antimicrobial activity