한국 성인 분변 시료에서 분리한 비피더스균의 유전자 다양성 분석

• 영문명: Analysis of Genetic Diversity of Bifidobacterium spp. Isolated from Korean AdultsFecal Samples
• 발행기관: 대한약학회
• 저자명: 배학균(Hak Gyoon Bae) 김선옥(Sun Ok Kim) 박종선(Jong Sun Park) 강병용(Byung Yong Kang) 최성숙(Sung Sook Choi) 강진양(Chin Yang Kang) 하남주(Nam Joo Ha)
• 간행물 정보: 『약학회지』제48권 제1호 (2004년), 20~26쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2004.02.28

영문 초록

Twelve strains of Bifidobacterium spp. Were isolated from the feces of healthy Korean 20~30 years. The identification of genera from isolates were performed by the microscopic observation and fructose-6-phosphate phosphoketolase (F6PPK) activity, which is the key enzyme to distinguish the Bifidobacterium spp. From other anaerobic bacteria. To determine the antibacterial resistance patterns, minimum inhibitory concentration (MIC) of several antibiotics (including antituberculosis agents) was determined. Five of the isolates showed the high degree of resistance to vancomycin. To investigate the genetic diversity between isolates and type strain of Bifidobacterium spp. From KCTC, we performed the RAPD-fingerprinting. Using a total set of four primers, it is possible to distinguish the isolates and Bifidobacterium spp. From KCTC. Thus, Bifidobacterium strains isolated from our samples may be a new species or strains of Bifidobacterium genera, and have the potential as a probiotics.