산화적 스트레스상태에서 B16F10 Murine Melanoma 세포의 항산화효소 활성에 대한 흑축추출물의 효과

• 영문명: Effect of Pharbitidis Seed Extract on the Antioxidant Enzyme Activity in B16F10 Murine Melanoma Cells by Oxidative Stress
• 발행기관: 대한약학회
• 저자명: 김안근(An Keun Kim) 차은정(Eun Jung Cha)
• 간행물 정보: 『약학회지』제48권 제1호 (2004년), 93~98쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2004.02.28

영문 초록

The purpose of this study was to evaluate the effect of pharbitidis seed extract (PE) on antioxidant enzymes. The cytotoxlcities of PE were measured by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The change of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity assay were measured. The SOD activities by PE-treated groups were lower than control group's one. In the co-treated with hydrogen peroxide (H2O2) group, SOD activity was higher than H2O2 treated group's activity. In the case of GPx, GPx activities were increased in both PE-treated and co- treated with H202 group. In the case of CAT, H2O2 treated group's activityies were very increased. The CAT activities by PE-treated groups were lower than control group's one, but the activity of co-treated group with H202 was higher than that of control group's one. These results suggest that PE has antioxidant activity.

키워드

antioxidant enzyme activity pharbitidis seed ROS