페튜인에 대한 면역글로부린 Y의 생성 및 친화성 컬럼에 의한 분리

• 영문명: Production of Anti-fetuin Immnoglobulin Y and its Isolation Using an Affinity Column
• 발행기관: 대한약학회
• 저자명: 정병욱(Byung Wook Jung) 정영윤(Young Yun Chung) 김윤중(Youn Jung Kim) 박지훈(Jee Hun Park) 구완모(Wan Mo Koo) 김병수(Byung Su Kim) 김기돈(Ki Don Kim) 김하나(Ha Na Kim) 김하형(HaHyung Kim)
• 간행물 정보: 『약학회지』제47권 제6호 (2003년), 438~443쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2003.12.31

영문 초록

For the investigations of the usefulness of immunoglobulin Y (IgY), we attempted to produce and isolate IgY from egg yolk of white Leghorn hens immunized with fetuin as a model antigen. We used three methods to optimize the recovery of IgY: water dilution, polyethylene glycol, and carrageenan. The daily yield of anti-fetuin IgY from egg yolk was also examined, and it was isolated using a fetuin-affinity column at a yield of IgY of 2.7% from total IgY. Furthermore, peroxidase-labeled antifetuin IgY was prepared, and was used to determine the minimum sensitivity against fetuin, which was found to occur to a fetuin concentration of 1 ng/ml.

키워드

IgY production isolation sensitivity.