림포사이트의 증식과 대식세포의 활성화에 관한 린나린과 아세칠 린나린의 비교 연구

• 영문명: Comparison of Linarin and Its Acetylated linarin for the Cytotoxicity on the Proliferation of Lymphocytes and Activation of Macrophages
• 발행기관: 대한약학회
• 저자명: 한신하(Shinha Han) 신지혜(Jee-hae Shin) 소명아(Myung-a So) 한혜란(Hye-ran Han) 김민준(Min-joon Kim) 임동술(Dongsool Yim) 김경제(Kyungjae Kim)
• 간행물 정보: 『약학회지』제47권 제4호 (2003년), 234~238쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2003.08.30

영문 초록

Linarin is a main compound from Chrysanthemum zawadskii var, latilobum. However, the biological mechanisms of these activities are unclear, Because of this wide diversity of effects, it is believed that they may be exerted through pluripotent effectors of linarin. In our previous screening study, the effects of linarin on the mouse macrophages cell line, RAW 264.7 cells, were investigated. It was found that linarin could stimulate macrophages activation by the production of tumor necrosis factor (TNF). The linarin (6.25~12.5 μg/ml) inhibited the production of NO in LPS-activated RAW 264.7 cells and linarin became an useful candidates for the development of new drug to treat endotoxemia and inflammation accompanied by the overproduction of NO. However, linarin-treated total lymphocyte showed cytotoxicity in a dose dependent manner between 20 μg/ml and 40 μg/nl. In this study, linarin derivative (acetylated linarin) was synthesized in order to obtain less-cytotoxicity of linarin and evaluated for their in vitro cytotoxic activity against mouse total lymphocyte. There was no cytotoxic activity in a dose dependent manner (20-40 μ g/ml of acetylated linarin whereas linarin showed. The production of NO, however was not the case by this modified linarin. The cell morphological change was not significantly changed in response to acetylated linarin alone and these effects were potentiated by the addition of LPS. These results suggest that acetylated linarin may be developed to be a promising new drug candidate without cytotoxicity on the basis of its activity of macrophage activation.

키워드

Chrysanthemum zawadskii macrophage activation linarin acetylated linarin.