2-Bromoacetyl-6-metroxynaphthalene을 형광유도체화제로 HPLC와 SPE를 이용한 생약제제 중 Ursodeoxycholic acid의 정량

• 영문명: Determination of Ursodeoxycholic Acid in Crude Drug Formulations by HPLC and SPE Using Selective Pre-column Derivatization with 2-Bromoacetyl-6-methoxynaphthalene
• 발행기관: 대한약학회
• 저자명: 진창화(Chang Hwa Jin) 임수희(Su Hee Lim) 이기진(Ki Jin Lee) 심형섭(Hyeong Sup Sim) 조의환(Eui Hwan Cho) 염정록(Jeong Rok Youm)
• 간행물 정보: 『약학회지』제46권 제6호 (2002년), 392~397쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2002.12.31

영문 초록

A simple and sensitive high performance liquid chromatographic method to quantitate ursodeoxycholic acid in crude drug pharmaceuticals was investigated. Ursodeoxycholic acid react with 2-bromoacetyl-6-methoxynaphthalene (Br-AMN) in the presence of triethylamine to form highly fluorescent derivative. The derivatization procedure was performed at 70oC and completed within 30 min. The optima1 wavelength of the fluorescence detector are λex=300 nm and λex= 460 nm. The LOD of the ursodeoxycholic acid was 25 ng/ml based on the S/N=3, and the LOQ was 80 ng/ml based on S/N=10. Crude drug pharmaceuticals pretreated by solid phase extraction (Sep-pak C18 cartridge) which were shown very geod separation and recwery values for the compound.

키워드

Ursodeoxycholic acid 2-bromoacetyl-6-methoxynaphthalene HPLC solid phase extraction pre-column derivatization crude drug formulations.