벼 렉틴 유전자의 플로닝 및 대장균에서의 발현

• 영문명: Molecular Cloning and Expression of Rice pectin Escherichia coli
• 발행기관: 대한약학회
• 저자명: 홍성관(Sung Gwan Hong) 전상훈(Sang Hoon Jeon) 김하형(Ha Hyun Kim) 공광훈(Kwang Hoon Kong)
• 간행물 정보: 『약학회지』제46권 제4호 (2002년), 270~275쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2002.08.31

영문 초록

The lectin gene from rice was amplified by the polymerase chain reaction. The amplified DNA was inserted into the expression vector pET26b and expressed it as a fusion protein with polyhistidine sequences in Escherichia coli. The recombinant protein was produced by induction with 0.4 Mm isopropyl-β-D- thiogalactopyranoside at 37oC and purified by an immobilized metal affinity chromatography; The recombinant protein was found to have lectin activity by the hemag- glutination inhibition assay; The hemagglutination activity of the recombinant protein was optimal at pH 4.0-7.0 and was dependent on Ca2+ and Mn2+.

키워드

Lectin rice gene cloning expression in E.coli.