붉은 서나물 잎 (Erechitites hieracifolia Raf.)에서의 Polyphenoloxidase 활성측정 및 항산화효오 특성분석

• 영문명: Properties of Polyphenoloxidase and Antioxidant Enzymes in the Leaves of Erechitites hieracifolia
• 발행기관: 대한약학회
• 저자명: 김안근(Kim An Keun) 이상은(Lee Sang Eun) 김국환(Kim Kuk Hwan) 권영이(Kwon Young Ee)
• 간행물 정보: 『약학회지』제46권 제4호 (2002년), 237~241쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2002.08.31

영문 초록

Polyphenoloxidase activity (PPO) in the leaves of Erechitites hieracifolia was estimated by Warburg's manometric method. The emzyme was most reactive toward chlorogenic acid followed by caffeic acid. Biethyldithiocarbamate and potassium cyanide were shown powerful inhibition rate to the polyphenoloxidase from the leaves of Erechitites hieracifolia . We confirme d antioxidant activity of the leave s ofErechitites hieracifolia by DPPH (1,1- diphenyl -2-picrylhydrazyl) radical scavenging method. Electrophorectic isoenzyme banding patterns of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were observed by native PAGE. The correlation of PPO and antioxidant enzymes is not investigated yet. That is need to further study.

키워드

Polyphenol oxidase DPPH Superoxide dismutase Peroxidase Catalase Erechitites hieracifolia.