간섬유화(경화)를 유도한 실험동물에서 생약 추출물(LH)의 항산화와 항섬유화 효과 검색

• 영문명: The Screening of Antioxidant and Antifibrotic Effect 5:om Water Extracts of Herbs(LH) in Biliary Liver Fibrosis (Cirrhosis) Induced Rsts
• 발행기관: 대한약학회
• 저자명: 임진아(Jin A Lim) 김기영(Ki Young Kim)
• 간행물 정보: 『약학회지』제46권 제2호 (2002년), 129~136쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2002.04.30

영문 초록

The pathogenesis of cholestatic liver injury as well as the modulation of hepatic fibrogenesis is causally associated with involvement of reactive oxygen species (ROS) and free radical reactions. In this study; we investigated whether dried extracts of oriental medicinie(LH) have antiodxidant and antifibrotic effect under the biliary liver fibrosis(cirrhosis) condition. The female Sprague-Dawley rats were divided in six groups (Normal, N-LH, op-2, op-4, opLH-2, opLH-4) and were observed in 2 weeks or 4 weeks. Fer this purpose the rats were operated by bile duct ligation/scission (BDL/S), which induced to liver fibrosis and cirrhosis. After surgery; the prepared LH was administered p.o. 2 ml/day/rat in 2 weeks or 4 weeks for opLH groups. During the observation period, jaundices appeared in eyes, ears and tail of all BDUS operated rats. And at the time of sacrifice, cholestasis was observed in proximal bile duct, especially the color of bile julce and urine in opLH-4 group showed more clear than op- 2, op-4 and opLH-2 group. The value of clinical parameters and product of lipid peroxidation (MDA) in sera and the hydroxyproline (hype) content in liver tissue were significantly increased in all liver fibrosis (cirrhosis) developed rats (p<0.001∼ 0.05). Among the clinical parameters of sera, value of BUN, ALP in opLH-4 group showed significantly lower than in op-4 group (p <0.05, p<0.001). The content of hyp in opLH-2, opLH-4 group (478.0 +/- 134.3μg/g,897.5 ± 118.2μg/g) showed lower than in op-2, op-4 group (528.9 ± 220.7μg/g,1023.8 +/- 277.1μg/g) and then the value of MDA in opLH-4 was also significantly reduced to 59.4% of that in op-4 group (p<0.001). The histological change (bile duct proliferation, fibrosis, collagen bundle) was similarly observed in op-2 group and in opLH-2 group, but the weak fibrosis and bile duct proliferation were observed in opLH-4 group compared with in op-4 group. Our data indicate that the 4 weeks treatment with LH extract suppressed lipid peroxidation and inhibited fibrotic (cirrhosis) process, and expetimental cholestatic liver disease is associated with increased lipid peroxidation in BDL/S operated rats. Hence wr concluded that the measurement of MDA and hyp can be useful monitor for the screening of antioxidant and antifibrotic effect in ecperimental liver fibrosis(cirrhosis), and LH has been shown to have hepatoprotective effect, antifibrotic effect and antioxidant effect.

키워드

Liver fibrosis lipid peroxidation MDA antioxidant & antifibrotic effect.