백혈병세포주 Jurkat T 세포에서 합환피 Albizzia julibrissin 물 추출물의 아포토시으 유도 효과

• 영문명: Effect of the Water Extract of Albizzia julibrissin on Apoptotic Cell Death in the Human Leukemic Jurkat T Cell Line
• 발행기관: 대한약학회
• 저자명: 황상구(Sang Gu Hwang) 이형철(Hyung Chul Lee) 김춘관(Chun Kwan Kim) 김용익(Yong Ik Kim) 주성민(Sung Min Ju) 김원신(Won Sin Kim) 전병훈(Byung Hun Jeon)
• 간행물 정보: 『약학회지』제45권 제6호 (2001년), 730~738쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.12.31

영문 초록

Apoptosis is a morphologically and biochemically distinct form of cell death that occurs in many different cell types in a wide variety of organisms.Albizzia julibrissin belonging the family Leguminosae has been used for the treatment of contusion, sore throat, amnesia, and insomnia in oriental traditional medicine. This study investigates whether the water extract of A. julibrissin induce apoptotic cell death in Jurkat T-acute Iymphoblastic leukemia (ALL) cells. Jurkat cells were increased inhibitions of cell viability in a concentration-dependent manner by A. julibrissin. This herbal medicine also caused apoptosis as measured by cell morphology and DNA fragmentation. The capability of A. julibrissin to induce apoptosis was associated with proteolytic cleavage of specific target protein such as poly (ADP-ribose) polymerase (PARP) protein suggesting the possible involvement of caspases. Our result showed that Bcl-2 and Bax protein levels were not changed in all A. julibrissin-treated groups compared to control group. These results suggest that A. julibrissin-mediated apoptosis is independent with Bcl-2 related signaling pathway in this cells.

키워드

Albizzia julibrissin water extract acute lymphoblastic leukemia Jurkat T cells apoptosis.