Chirose hamster ovary 세포에서 발현된 preS2 및 S부위 함유 HBsAg의 항체유발능

• 영문명: Antibody productivity of HBsAg containing both preS2 and S regions expressed in Chinese hamster ovary cells
• 발행기관: 대한약학회
• 저자명: 정성균(Sung Kyun Chung) 박정민(Jung Min Park) 이상봉(Sang Bong Lee) 박동우(Dong Woo Park) 김동연(Dong Yeon Kim) 김기호(Ki Ho Kim) 김홍진(Hong Jin Kim)
• 간행물 정보: 『약학회지』제45권 제6호 (2001년), 708~714쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.12.31

영문 초록

Many studies have provided evidences that hepatitis B surface antigen (HBsAg) including pres region could be an ideal candidate for a new hepatitis B virus (HBV) vaccine with higher efficacy. We established CHO cell lines, H-CHO-2 and IY-CHO-11 expressing high levels of HBsAg containing preS2 and S pretein by stable transfection method. These cell lines expressed the correct size (about 1 kb in length) of HBsAg myNA as eld)ected. The purified protein from the culture supernatants of the clones showed the same sizes as those expressed in native hepatitis B virus (24 kDa, 27 kDa, 34 kDa and 36 kDa). Antibody productivity of CHO-derived HBsAg protein at lower dose challenge was higher than the protein containing S region alone expressed in yeast system. These results indicate that CHO-derived HBsAg protein containing preS2 and S region can be effectively used for a better immune response as a HBV vaccine.

키워드

Hepatitis B virus vaccine CHO.