Streptomyces aburabiensis SMF30이 생산하는 Cathepsin B 저해물질의 발효생산 및 특성분석

• 영문명: Production and Physico-chemical Properties of Cathepsin B Inhibitor from Streptomyces aburabiensis SMF30
• 발행기관: 대한약학회
• 저자명: 최영출(Young Chool Choe) 김인섭(In Seop Kim) 박상진(Sang Jin Park) 윤성준(Sung Joon Yoon) 이계준(Kye Joon Lee)
• 간행물 정보: 『약학회지』제39권 제3호 (1995년), 306~313쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1995.06.30

영문 초록

The aim of the present study was to produce low molecular weight cathepsin B inhibitor. A strain of Streptomyces aburabiensis isolated from soil in Korea was selected and the optimum condition for the production of the inhibitor was evaluated. Glucose and soytone were selected as best carbon and nitrogen sources, respectively. From the kinetic analysis in batch fermentation, it was found that the specific cathepsin B inhibitor production rate (qp) was linearly related to specific growh rate (mc). The inhibitor in culture filtrate was purified by adsorption on activated charcoal, butanol extraction, silica gel chromatography, ion exchange chromatography using Dowex-1 (C1- form) and Amberlite IRC-50 (H+ form), and preparative TLC. From the UV, IR, Mass spectroscopy and 1H-NMR. the inhibitor was thought to be a new inhibitor of which molecular weight was 199.