표고버섯 렉틴의 림프구 자극 분열 및 암 세포 응집 효과

• 영문명: Mitotic Stimulation and Cancer Cell Agglutination of the Lectin from Lentinus edodes
• 발행기관: 대한약학회
• 저자명: 문익재(Ik Jae Moon) 정시련(See Run Chung) 전경희(Kyung Hee Jeune)
• 간행물 정보: 『약학회지』제39권 제3호 (1995년), 260~267쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1995.06.30

영문 초록

A lectin from the edible mushroom, Lentinus edodes, was purified through physiological saline extraction, ammonium sulfate fractionation and column chromatographies. On polyacrylamide gel electrophoresis, 0.05M fraction from hydroxyapatite column exhibited adjacent four sharp bands. The partially purified lectin agglutinated the erythrocytes of rabbit, mouse and rat, but did not agglutinate human erythrocytes. The lectin''s mitogenic effects were tested by its application to human and murine splenic lymphocytes. The results showed that the 0.05M fraction from hydroxyapatite was mitogenic, and the optimal dose of Lentinus edodes lectin was slightly lower than Con A by the culture with murine splenic and human peripheral lymphocytes. Meanwhile, its ability to agglutinate transformed cells was tested by its administration to continuous cell lines L1210 and HeLa cells. The lectin was found to be an agglutinin of tumor cell lines tested by L1210 and HeLa cells.