신장 조직의 브라디키닌 결합부위 발현

• 영문명: Expression of Bradykinin Binding Sites within the Mammalian Kidney Tissues
• 발행기관: 대한약학회
• 저자명: 정성현(Sung Hyun Chung) 정지창(Jee Chang Jung)
• 간행물 정보: 『약학회지』제38권 제5호 (1994년), 602~607쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.10.30

영문 초록

Based upon the previous experiments showing that kidney and lung tissues of rat had relatively abundant bradykinin binding sites, we tried to characterize and determine the densities of the bradykinin binding sites in the rabbit kidney tissue and proximal tubular cells under different growing conditions. Among the kidney tissue renal medulla segments showed the highest bradykinin binding sites. To determine which growth factors are to add in the serum free culture medium to express selectively the bradykinin binding sites in the rabbit kidney proximal tubular cells, we tried so called hormone-deletion approach and in here insulin, hydrocortisone, transferrin, triiodothyronine and prostagiandin E1 are examined. By performing receptor binding assay and determination of protein concentrations, we may conclude that the most required hormones in the expression for bradykinin binding sites are insulin and transferrin, and fetal bovine serum is shown to be less effective in this regard.