다제내성 황색포도상구균이 가지고 있는 클로람페니콜 내성 플라스미드의 동정

• 영문명: Characterization of Chloramphenicol Resistant Plasmid of Multidrug-resistant Staphylococcus aureus
• 발행기관: 대한약학회
• 저자명: 이대운(Dae Woon Lee) 문경호(Kyung Ho Moon)
• 간행물 정보: 『약학회지』제37권 제6호 (1993년), 621~624쪽, 전체 4쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1993.12.30

영문 초록

The clinical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloroamphenicol, clindamycin. erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.14kb plasmid(pKH7) encoded resistance to chloramphenicol. The cleavage map of pKH7 was determined by restriction enzyme mapping techniques. The cleavage map is given for BstEII, HindIII, HpaII, and XbaI. The above restriction endonucleases have a single site, but nucleases BamHI, BglI, BglII, EcoRI, EcoRV, HaeIII, HpaI, KpnI, PstI, PvnIl, SalI, SmaI, and XhoI have no site on this plasmid.

키워드

Multidrug-resistant Staphylococcus aureus protoplast transfomation chloramphenicol resistance plasmid restriction map.