Alkaline phosphatase의 기질 특이성

• 영문명: Substrate Specificity of Alkaline Phosphatase
• 발행기관: 대한약학회
• 저자명: 이경태(Kyung Tae Lee) 권창호(Chang Ho Kwon) E. Waelens W. Merlevede
• 간행물 정보: 『약학회지』제37권 제6호 (1993년), 571~576쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1993.12.30

영문 초록

The substrate specificity of the purified rabbit plasma alkaline phosphatase (ALPase) was determined towards a extended range of potential substrates including relatively simple phosphate derivatives as p-NPP and indolyl phosphate, and several synthetic peptides and phosphoproteins. These results further estabilish the broad substrate specificity of these circulating enzymes. Interestingly, the plasma ALPase preferentially dephosphorylates Thr over Ser residues, as demonstrated with a series of synthetic peptides. The latter result is in contradiction to the behaviour of the tissue ALPase, which is thought to the ultimate source of plasma ALPase, and open therefore new perspectives with respective to the origin and "solubilisation" processes of these enzymes. Dephsphrylation of protein substrates by endogenous and isolated plasma ALPases indicates that ALPase probably displays protein phosphatase activity in vivo.

키워드

Alkaline phosphatase Phosphotyrosine Phosphatase substrate specificity p-NPP.