티미딜산 생성효소 활성이 높은 메토트렉세이트-내성 균주의 검색

• 영문명: Screening of Methotrexate-Resistant Strains with High Thymidylate Synthase Activity
• 발행기관: 대한약학회
• 저자명: 곽인영(In Young Kwak) 이종수(Jong Soo Lee)
• 간행물 정보: 『약학회지』제36권 제4호 (1992년), 345~349쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1992.08.30

영문 초록

Thymidylate synthase activity from extracts of various methotrexate-resistant strains was measured by spectrophotometric assay. Methotrexate-resistant strains of Lactobacillus, Pseudomonas sp., Micrococcus sp. HS-1, Klebsillela pneumonae, Cellulomonas fimi and Serratia marcescens elevated thymidylate synthase levels, especially, Pseudomonas sp. KL-9 resistant to 10-9M methotrexate have a 156-fold increase in thymidylate synthase, which suggests that Pseudomonas sp. is a convenient source of thymidylate synthase. Several methotrexate strains of yeast were tested, however, their enzyme activity was generally lower than that of bacteria tested.