흰쥐의 장내미생물로부터 분리한 새로운 페놀 설포트란스페라제

• 영문명: Novel Phenol Sulfotransferase of Klebsiella K-36, Rat Intestinal Bacteria
• 발행기관: 대한약학회
• 저자명: 김형수(Hyung Soo Kim) 김동현(Dong Hyun Kim)
• 간행물 정보: 『약학회지』제36권 제2호 (1992년), 167~172쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1992.04.29

영문 초록

Klebsiella K-36producing novel sulfotransferase was isolated from rat intestinal flora. The novel sulfotransferase catalysed the transfer of sulfate group from p-nitrophenylsulfate to phenolic compounds but it did not use PAPS(3''-phosphoadenosine 5''-phosphosulfate) as a donor substrate. The present enzyme was 160K daltons. Optimal pH was 10. When p-nitrophenyl sulfate was used as a donor substrate, 1-naphthol was the best substrate, followed by phenol, phenanthrol and tyrosine, The apparent Km for phenol using p-nitrophenylsufate as a donor substrate and that for p-nitrophenylsulfate using pheol as an acceptor substrate were determined to be 0.66mM and 0.11mM, respectively.