혈청 3,5,3''-Triiodothyronine 측정을 위한 효소-면역 분석의 개발 연구

• 영문명: Development of Homogeneous Enzyme Immunoassay for Serum 3,5,3''-Triiodothyronine Determination
• 발행기관: 대한약학회
• 저자명: 이희주(Hee Joo Lee)
• 간행물 정보: 『약학회지』제27권 제2호 (1983년), 117~124쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1983.06.30

영문 초록

For development of EMIT-T3 assay, the conjugation of 3, 5, 3''-triiodothyroformic acid NHS ester to G6PDH was attempted in various reaction conditions. Up to now, the best conjugation condition was the ratio of T3-NHS:G6PDH=100 in 25% carbitol-Tris buffer at pH 9, OoC during overnight. The obtained T3-G6PDH conjugates usually had 20% residual enzyme activity which was inhibited by 40~70% with various anti-T3 antibodies. Utilizing the conjugate I and an antibody (S2633G), a useful standard curve for T3 assay was obtained in the range of 0 to 5ng/ml with 499 EMIT units of separation.