LC-MS/MS를 이용한 당귀수산 추출물 중 17종 성분의 함량분석

• 영문명: Quantitative Analysis of the Seventeen Marker Components in Dangguisu-san Using Ultra-performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry
• 발행기관: 대한약학회
• 저자명: 서창섭 신현규
• 간행물 정보: 『약학회지』제58권 제3호 (2014년), 158~164쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2014.06.30

영문 초록

Dangguisu-san is a well-known traditional Korean herbal medicine prescription and has been widely used to treat ecchymosis, blood stagnation, and pain resulting from physical shock in Korea. In this study, an ultra-performance liq-uid chromatography-electrospray ionization-mass spectrometer method was established for the simultaneous determination of the 17 biomarker components in Dangguisu-san. All analytes were separated on an UPLC BEH C18 (100×2.1 mm,1.7 μm) column and maintained at 45 oC. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in water(A) and acetonitrile (B) by gradient flow. The injection volume was 2.0 μl and the flow rate was 0.3 ml/min with detection at mass spectrometer. Calibration curves of the 17 biomarker components were acquired with r 2 values ≥0.9951. The values of limit of detection and quantification of all analytes were 0.02~6.32 ng/ml and 0.05~18.95 ng/ml, respectively. The amounts of the 17 components in Dangguisu-san sample were 3.17~13,224.50 μg/g.

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키워드

LC-MS/MS Dangguisu-san quantitative analysis