Methionine- R-reductase plays a defensive role against cadmium toxicity in Schizosaccharomyces pombe

• 발행기관: 강원대학교 기초과학연구소
• 저자명: Hannah Jo Kisup Ahn Chang-Jin Lim
• 간행물 정보: 『기초과학연구』제23집, 33~44쪽, 전체 12쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2012.12.31

영문 초록

Methionine sulfoxide reductases (Msrs) are oxidoreductases which catalyze thiol-dependent reduction of free and protein- bound methionine sulfoxides back to corresponding methionine. Methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO) are reduced by methionine-S-sulfoxiede reductase (MsrA) and methionine-R-sulfoxide reductase (MsrB), respectively. In the present study, the cellular functions of MsrB were further characterized using the MsrB-overexpressing recombinant plasmid pFMetSO in the fission yeast. The S. pombe cells harboring pFMetSO exhibited better growth than the vector control cells, when shifted into the fresh medium containing cadmium. In the presence of different levels of cadmium, the S. pombe cells harboring pFMetSO contained diminished NO and reactive oxygen species (RS) levels than the vector control cells. On the contrary, the S. pombe cells harboring pFMetSO contained higher levels of total glutathione (GSH) than the vector control cells under the same treatments. Taken together, the S. pombe MsrB plays a protective role against cadmium toxicity.

목차

Abstract

1. Introduction

2. Materials and Methods

3. Results and Discussion

4. Acknowledgment

5. References