토양에서 분리된 Chitin 분해성 Pseudomonas acidovorans KM-3의 분리 및 특성

• 영문명: Isolation of Chitinolytic Pseudomonas acidovorans from soil and it’s properties
• 발행기관: 대전대학교 환경문제연구소
• 저자명: 이찬용(Chan-Yong Lee)， 김광엽(Kwang-Yup Kim)
• 간행물 정보: 『環境問題硏究所 論文集』第3號, 3~17쪽, 전체 15쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1998.02.28

영문 초록

For the production of potent chitinolytic enzyme from bacteria. screening was carried out. Of 100 samples from soil of Kyung-gi and Chung-cheong area. 7 strains of chitinolytic bacteria were isolated. Among them KM-3 was identified as Pseudomonas acidovorans KM-3 which showed the higher chitinolytic activity than others. Culture conditions of Pseudomonas acidovorans KM-3 for the production of chitinolytic enzyme were inverstigated and the enzyme was partially purified by the ammonium sulfate fractionation. Maximum production of chitinolytic enzyme was obtained at pH 7.0 and 30℃ with chitin concentration between 0.2% and 1.0%. Conditions for the enzyme production were optimized using Jar-fermentor. Maximum enzyme activity was shown as 4.25 units/ml at the 24hours cultivation. Optimum temperature and pH of crude enzyme was 40℃ and pH 7.0. respectively. It could be applied to waste-treatment for the chitinous substrates.

목차

ABSTRACT

Ⅰ. 서론

Ⅱ. 실험 재료 및 방법

Ⅲ. 결과 및 고찰

Ⅳ. 결론

〈감사의 글〉

참고문헌

키워드

Pseudomonas acidovorans chitinase inducing enzyme