Cytoprotective Effects of Serum Hormone Deprivation against Glutamate Toxicity in HT22 Mouse Hippocampal Cells

• 발행기관: 대한해부학회
• 저자명: Eunyung Son Dong Hoon Lee Gu Seob Roh Hyun Joon Kim Sang Soo Kang Gyeong Jae Cho Wan Sung Choi
• 간행물 정보: 『대한해부학회지』제41권 제3호, 205~211쪽, 전체 7쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.06.30

영문 초록

Reactive oxygen species (ROS) are important signaling molecules or mediators in many cellular responses, including the oxidative-burst defense response. Certain hormones are neuroprotective because they are modulators of neuronal activity or ROS scavengers. We have examined the effect of a hormone-free condition on ROS levels following glutamate-induced excitotoxicity in the mouse hippocampal HT22 cell line. We show that hormone starvation slightly elevates ROS and that continuous low concentrations of ROS induce expression of antioxidant enzymes, such as heme oxygenase-1 (HO-1). In addition, N-acetyl-L-cysteine (NAC) restores the expression of ERK1/2 protein in hormone-starved HT22 cells. These findings suggest that whereas high-dose ROS are cytotoxic and lead to tissue damage in the brain low-dose ROS may act in neuroprotective signaling.

키워드

Reactive oxygen species Glutamate Cytotoxicity HT22 cells