- 영문명
- Development of ELISA-kit of Quantitative Analysis for Adiponectin and Their Correlation with Cardiovascular Risk Factors
- 발행기관
- 한국역학회
- 저자명
- 지선하(Sun Ha Jee) 이승복(Seungbok Lee) 민성식(Sungsik Min) 박정용(Jungyong Park) 김현숙(Hyon-Suk Kim) 김상연(Sang Yeun Kim) 윤지은(Ji Eun Yun) 이선주(Sun Ju Lee) 지은정(Eun Jung Jee) 이희연(Hee Yeon Lee) 송혜윤(Hye Yun Song)
- 간행물 정보
- 『한국역학회지』韓國疫學會誌 第29卷 第2號, 165~175쪽, 전체 12쪽
- 주제분류
- 의약학 > 면역학
- 파일형태
- 발행일자
- 2007.12.30
국문 초록
영문 초록
Purposes: To develop a rapid, sensitive, qualitative ELISA-kit for serum adiponectin and examine correlation with adiponectin and cardiovascular risk factors.
Methods: On the base of monoclonal antibodies against adiponectin, apply indirect ELISA to study the performance parameter of the kit. The correlation was examined between adiponectin and cardiovascular risk factors including waist circumference, body mass index, triglyceride, and HDL cholesterol.
Results: The limited concentration of detection of the ELISA-kit was 1㎍/㎖. Linearity with R&D system and AdipoGen with this ELISA-kit was acceptable: the linear equation with R&D system was y=1.0116x+0.4629 (R2=0.97) and linear equation with AdipoGen was y=0.9562x+1.1961 (R2=0.93), respectively. The average recovery rate of the ELISA-kit ranged 92 to 104%. The correlation coefficient of waist circumference with adiponectin was -0.2276 (p＜0.0001) among men and -0.2328 (p＜0.0001) among women.
Conclusion: This ELISA-kit was quick, sensitive, and stable and can be used to determine adiponectin in serum.
Methods: On the base of monoclonal antibodies against adiponectin, apply indirect ELISA to study the performance parameter of the kit. The correlation was examined between adiponectin and cardiovascular risk factors including waist circumference, body mass index, triglyceride, and HDL cholesterol.
Results: The limited concentration of detection of the ELISA-kit was 1㎍/㎖. Linearity with R&D system and AdipoGen with this ELISA-kit was acceptable: the linear equation with R&D system was y=1.0116x+0.4629 (R2=0.97) and linear equation with AdipoGen was y=0.9562x+1.1961 (R2=0.93), respectively. The average recovery rate of the ELISA-kit ranged 92 to 104%. The correlation coefficient of waist circumference with adiponectin was -0.2276 (p＜0.0001) among men and -0.2328 (p＜0.0001) among women.
Conclusion: This ELISA-kit was quick, sensitive, and stable and can be used to determine adiponectin in serum.
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연구 결과
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