High-Resolution Melting Assay for the Detection of fliC variations in Salmonella serotypes Dublin and Naestved

• 영문명: High-Resolution Melting Assay for the Detection of fliC variations in Salmonella serotypes Dublin and Naestved
• 발행기관: 고려대학교 보건과학연구소
• 저자명: 유영재(YoungJae Yoo) 권은혜(EunHae Kwon) 김슬기(SulKi Kim) 김희영(HeeYoung Kim) 이현정(HyunJeong Lee) 최정훈(JeongHoon Choi) 최현주(HyunJu Choi) 황영진(YoungJin Hwang) 임영희(YoungHee Lim)
• 간행물 정보: 『보건과학논집』保健科學論集 Vol.32 No.2, 67~72쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2006.12.01

영문 초록

  High resolution melt curve analysis(HRM) is a simple post-PCR technique which can be used for mutation scanning and genotyping. We identified Salmonella enterica Dublin and S. enterica Naestved which have single base difference between them in fliC gene by HRM. The 102-base pair amplicons expressed different melting temperatures. Using the method, we can identify serotypes even which have a base variant without post-PCR handling, resulting in short analysis time.

목차

Abstract
Ⅰ. Introduction
Ⅱ. Materials and Methods
Ⅲ. Results and Discussion
References

키워드

High resolution melt curve analysis genotyping Salmonella