폐쇄성 수면무호흡증환자의 혈장 단백에 대한 프로테옴 분석

• 영문명: Proteomic analysis of plasma proteines from patients with Obstructive sleep apnea
• 발행기관: 고려대학교 보건과학연구소
• 저자명: 이승관(SeungGwan Lee) 이창규(ChangKyou Lee) 윤대위(DaeWe Yoon) 성은미(EuenMe Sung) 김진관(JinKwan Kim) 신철(Chol Shin) 이성호(SungHo Lee)
• 간행물 정보: 『보건과학논집』保健科學論集 Vol.29 No.2, 45~50쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2003.12.01

영문 초록

  Obstructive Sleep Apnea(OSA) is characterized by episode of complete or partial pharyngeal obstruction during sleep and deeply related to cardiovascular disease and cerebrovascular disease. Many studies show that Hypoxia, Hypertension, and the production of superoxide produced by elevated homocysteine concentration may damage to vascular endothelium. In this study we performed proteomic-based approach to characterize the plasma protein associated with wound healing mechanism. First, overnight polysomnographic study was carried out in sleep laboratory, using standard recording techniques. Five cases of OSA patients and 5 normal controls were analyzed by two dimensional gel electrophoresis (pH 3-10NL, 8-18% gradient). Protein spots were cut from 2 -D gels stained by Coomasie blue, and were identified by using matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS). The results were compared with those of paired normal controls. Comparative analysis of the respective spot patterns in 2DE shows that Vitronectin which plays an important role in repairing wounded vessel was increased. Following protein was also increased in OSA patients : Ig alpha-1 chain C region

목차

Abstract
Ⅰ. Introduction
Ⅱ. Materials and Method
Ⅲ. Results and Discussion
Ⅳ. References