A Single-Step Multiplex RT-PCR to Detect Enteric Viruses

• 영문명: A Single-Step Multiplex RT-PCR to Detect Enteric Viruses
• 발행기관: 고려대학교 보건과학연구소
• 저자명: 임영희(YoungHee Lim) 허정원(JeongWeon Huh) 남용석(YongSuk Nam)
• 간행물 정보: 『보건과학논집』保健科學論集 Vol.31 No.2, 85~90쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2005.12.01

영문 초록

  A single-step multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay that detects and identifies Adenovirus, Astrovirus, Norovirus genogroup I (GI) and II (GII), and Rotavirus in stool samples is described. The primers for Adenovirus, Norovirus GI and GII, and Rotavirus were designed from the capsid region of target viral genome and one primer set for Astrovirus was designed from the RNA-dependent RNA polymerase gene. The multiplex RT-PCR reaction mixture containing five sets of primers amplified each expected product independently and specifically in a single-step with the One Step RNA PCR Kit (AMV) (Takara). The multiplex RT-PCR was validated against simplex RT-PCR for Astrovirus, Norovirus GI, Norovirus GII, and Rotavirus, and against simplex PCR for Adenovirus. The multiplex RT-PCR was equally specific in detecting Adenovirus, Astrovirus, Norovirus GI and GII, and Rotavirus compared with simplex RT-PCR and PCR.

목차

Abstract
Ⅰ. Introduction
Ⅱ. Materials and Methods
Ⅲ. Results and Discussion
References

키워드

Enteric Viruses Multiplex RT-PCR