Comparative analysis of two thioredoxins from Schizosaccharomyces pombe

• 영문명: Comparative analysis of two thioredoxins from Schizosaccharomyces pombe
• 발행기관: 강원대학교 기초과학연구소
• 저자명: Youn Hee Shin Young-Wook Cho Hang-Lim Jung Chang-Jin Lim
• 간행물 정보: 『기초과학연구』제11집, 134~146쪽, 전체 13쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2000.12.01

영문 초록

Eukaryotic thioredoxins (TRXs) have been implicated in a wide variety of biochemical and physiological functions. Previously, a genomic DNA and a cDNA encoding thioredoxin were cloned from Schizosaccharomyces pombe. However, C-terminal coding region and downstream region were found to be different, when compared with the two nucleotide sequences. The putative C-terminal region of TRX (called C-type) estimated from the cDNA contains -V-R-L-N-R-S-L, whereas that of TRX (called G-type) predicted from the genomic DNA, contains -A-S-I-K-A-N-L. The plasmid pYJKI which codes for the G-type TRX restored normal growth in the mutant E. coli strain, whereas the plasmid pYM51 which codes for the C-type TRX didn't. The C-type TRX is able to act as a subunit of T7 DNA polymerase, while the G-type TRX is not. Both types of TRX are able to act as a cofactor of methionine sulfoxide reductase and are successfully used for the assembly mechanism of filamentous phages. Due to the differences in the C-terminal amino acid sequences of two S. pombe TRXs, they show some differences in case of growth complementation and T7 phage growth.

목차

1. Introduction

2. Material and Method.

3. Result and Discussion

4. References