Electroporation에 의한 벼 현탁배양 세포의 형질전환

• 영문명: Transformation of Rice Suspension-Cultures by Electroporation
• 발행기관: 강원대학교 기초과학연구소
• 저자명: 최성진(Seong Jin Choi) 김준철(Joon Chul Kim)
• 간행물 정보: 『기초과학연구』제8집, 285~295쪽, 전체 11쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 1997.12.01

영문 초록

Calli were induced from the leaf base region of germinated rice (Oryza sativa L. cv Nakdong) on LS medium supplemented with 2.5mg/L 2,4-D in the dark at 27℃. Embryogenic calli of pale yellow and globular type were selected and used for the initiation of cell suspension cultures is AA₂ liquid medium with 2mg/L 2,4-D, 0.2mg/L kinetin and 0.1mg/L GA₃ For rice transformation, suspension-cultured cells were enzymatically macerated for 2 hours in 2% Cellulase solution and filtered through 520, 380, 230 and 90㎛ stainless mesh. Filtered embryogenic microcolonies of 90-230/㎛ with pBl121 were electroporated at 400 V/cm for 1 ms. Transformed calli were selected from the electroporated calli on 100mg/L kanamycin-containing media. GUS gene in the genomic DNA of 5 putative transformed callus lines were detected by PCR. The expression of GUS gene in the kanamycin-resistance calli was confirmed by spectrophotometric assay and histochemical assay of GUS activity. Regenerants from the kanamycin-resistance calli showed high specific expression of GUS gene.

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