무(Raphanus sativus)의 하배축 유래 캘러스로부터 식물채 재분화

• 발행기관: 강원대학교 기초과학연구소
• 저자명: 박민철 손수인 김준철
• 간행물 정보: 『기초과학연구』제8집, 276~284쪽, 전체 9쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 1997.12.01

영문 초록

Hypocotyl explants of radish (Raphanus sativus L. cv F, Taewang) formed calli when cultured on MS medium supplemented with 2.0 mg/L 2, 4-D, 0.05mg/L kinetin, and 0.5 mg/L BAP. Calli were subcultured for four months at intervals of four weeks and then transferred to media containing various concentrations of IAA, BAP, kinetin and zeatin for shoot organogenesis. Shoot primordia were fomed on Ms medium with the combinations of 2 mg/L IAA and Img/L BAP, 21 mg/L IAA and 6-7mg/L kinetin, and 2mg /L IAA and 1-2mg/L zeatin but regenerated normal shoots were produced only upon transfer to medium containing 2mg/L IAA and 7mg/L kinetin. Excised shoots were rooted when cultured on MS basal medium after one week of culture. Regenerants were successfully transplanted to potting soil and grown to maturity in a growth chamber. Histological observations of shoot forming callus revealed that shoot primordia were initiated with the formation of smaller and isodiametric cell files, and that merismetic activity preceded organogenesis.

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