Isolation and Characterization of Three Homeodomain-Leucine Zipper Genes, Phz1, Phz2, and Phz4 from Pimpinella brachycarpa Shoot Tips

• 영문명: Isolation and Characterization of Three Homeodomain-Leucine Zipper Genes, Phz1, Phz2, and Phz4 from Pimpinella brachycarpa Shoot Tips
• 발행기관: 강원대학교 기초과학연구소
• 저자명: Yong-Hwan Moon Sang-Bong Choi Jong-Il Kim Joon-Chul Kim Tae-Jin Han 외 2인
• 간행물 정보: 『기초과학연구』제8집, 227~237쪽, 전체 11쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 1997.12.01

영문 초록

By screening a Pimpinella brachycarpa shoot tip cDNA library with the soybean homeo-domain-leucine zipper (HD-Aip) gene, Gmhl, as a probe, we isolated three cDNA clones, Phz1, Phz2, and Phz4, encoding polypeptides that contain a homeodomain and a closely linked leucine zipper motif. In both nucleotide and deduced amino acid sequences, Phz1, Phz2, and Phz4 were highly homologous to two Arabidopsis thaliana HD-Zip genes, a soybean HD-Zip gene and a tomato HD-Zip gene in an HD-Aip motif. Genomic Southern blot analysis indicated that Phz2 and Phz4 are members of a multigene family. RNase protection assay revealed that Phz2 and Phz4 are expressed at all organs including leaves, petioles, roots and shoot tips. So far, these HD- Zip proteins have been found only in dicotyledonous plants. Thus, it may be speculated that Phz1, Phz2, and Phz4 proteins could impart functional characters unique to the dicotyledonous plants that would express them in processes common to those plants.

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Materials and Methods

Results and Discussion

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