참외 전장유전체 염기서열 분석 및 SSR 마커 개발

• 영문명: Whole genome re-sequencing and development of SSR markers in oriental melon
• 발행기관: (사)한국식물생명공학회
• 저자명: 송운호(Woon-Ho Song) 정상민(Sang-Min Chung)
• 간행물 정보: 『Journal of Plant Biotechnology』46권 2호, 71~78쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.06.30

영문 초록

The objective of this study was to use ‘Danta PR’, NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, ‘Hyangseo 1’ and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of ‘Danta PR’ BC1 (F1 x ‘Danta PR’) population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties.

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재료 및 방법

결 과

결 론

키워드

Indel Oriental melon Polymorphic markers Resequencing SSR