Agrobacterium에 의한 오이 형질전환에서 자엽절 절편의 이용

• 영문명: The use of cotyledonary-node explants in Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.)
• 발행기관: (사)한국식물생명공학회
• 저자명: 장현아(Hyun A Jang) 김현아(Hyun A Kim) 권석윤(Suk Yoon Kwon) 최동욱(Dong Woog Choi) 최필선(Pil Son Choi)
• 간행물 정보: 『Journal of Plant Biotechnology』38권 3호, 198~202쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.09.30

영문 초록

Agrobacterium tumefaciens-mediated cotyle-donary-node explants transformation was used to produce transgenic cucumber. Cotyledonary-node explants of cucumber (Cucumis sativus L. cv., Eunsung) were co-cultivated with Agrobacterium strains (EHA101) containing the binary vector (pPZP211) carrying with CaMV 35S promoter-nptII gene as selectable marker gene and 35S promoter-DQ gene (unpublished data) as target gene. The average of transformation efficiency (4.01%) was obtained from three times experiments and the maximum efficiency was shown at 5.97%. A total of 9 putative transgenic plants resistant to paromomycin were produced from the cultures of cotyledonary-node explants on selection medium. Among them, 6 transgenic plants showed that the nptII gene integrated into each genome of cucumber by Southern blot analysis.

키워드

Agrobacterium cotyledonary-node paromomycin transgenic cucumber