황금배(Pyrus pyrifolia Nakai) 잎 조직으로부터 기내 신초 재분화

• 영문명: In vitro shoot regeneration from Leaf tissue of Whangkeumbae pear (Pyru pyrfolia Nakai)
• 발행기관: (사)한국식물생명공학회
• 저자명: 천재안(Jae Au Chun) 도경란(Kyung Ran Do) 김세희(Sc Hec Kim) 조강희(Kang Hee Cho) 김현란(Hyun Ran Kim) 황해성(Hae Sung
• 간행물 정보: 『Journal of Plant Biotechnology』39권 4호, 288~294쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.12.31

영문 초록

In order to establish an efficient adventitious shoot regeneration conditions from leaf explants for Asian pear ``Whangkeumbae``, the effect of concentration and kinds of plant growth regulator and carbon source was investigated. Leaf explants of cultures grown on Murashige and Skoog (MS) medium containing 8 g/L plant agar were used. When the medium contained 0.25 mg/L thidiazuron (TDZ) and 0.3 mg/L indolebutyric acid (IBA), the adventitious shoot regeneration rate (ASRR) was greater as 61.1% than others treated and higher TDZ concentrations (2.5 and 5 mg/L) treatment significantly reduced the ASRR. As the effect of IBA and indoleacetic acid (IAA) concentration on the ASRR, 0.5 mg/L TDZ plus different concentration of IAA exhibited relatively high ASRR and 0.5 mg/L TDZ pIus 0.3 mg/L IAA showed the highest ASRR of 76.7%. Also the effect of sucrose and sorbitol as carbon source on regeneration was examined. The highest ASRR and the most shoots per explants averaged 94.4% and 3.49 by treatment of 30 mg/L sorbitol, respectably. Sorbitol is considered better carbon source than sucrose for shoot regeneration of ``Whangkeumbae`` pear.