탄소급원처리에 의한 기내 더덕 식물체의 비대근 형성

• 영문명: Microtuber Formation from In Vitro Codonopsis lanceolata Plantlets by Sugar
• 발행기관: (사)한국식물생명공학회
• 저자명: 최용의(Yong Eui Choi) 김지아(Ji Ah Kim) 문홍규(Heung Kyu Moon)
• 간행물 정보: 『Journal of Plant Biotechnology』40권 3호, 147~155쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.09.30

영문 초록

In this experiment, we report for the first time mass propagation by in vitro mircrotuberization of Codonopsis lanceolata. We first examined the effect of cytokinins on multiple shoot induction. 2.0 mg·L-1 of kinetin not only gave the highest rate of shoot induction (19.1%) but also the elongation of shoot (17.1 mm). Secondly, we investigated the effect of sugars on in vitro microtuberization from nodal segments. The diameter of tuberous roots was enlarged in the half-strength MS medium supplemented with 145.9 mM sucrose. Histological analysis revealed that the number of parenchymatous cell containing starch grains increased in the tuberous roots. In addition, unlike in non-tuberous root, vascular bundles were scattered inner cortex layer. Thirdly, in order to preserve and stimulate the germination, microtubers were stored at 4℃ refrigerator during 9 months and then transplanted to the artificial soils (vermiculrite : peatmoss = 1:1 v/v), resulting that the rates of survival and germination were 75% and 70%, respectively. These results indicated that mass propagation of C. lanceolata was achieved by in vitro microtuber formation, suggesting that this protocol might be applied for not only the propagation of elite clones but also conservation of C. lanceolata germplasm.

키워드

Codonopsis lanceolata Germplasm Microtuber Mircrotuberization Tuberous root