Evaluation on Antimicrobial Activity of Psoraleae semen Extract Controlling the Growth of Gram- Positive Bacteria

• 발행기관: 한국축산식품학회
• 저자명: Sejeong Kim Soomin Lee Heeyoung Lee Jimyeong Ha Jeeyeon Lee Yukyung Choi Hyemin Oh Joonbae Hong Yoh
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제37권 제4호, 502~510쪽, 전체 9쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2017.08.31

영문 초록

This study investigated bacterial growth-inhibitory effect of 69 therapeutic herbal plants extracts on 9 bacterial strains using a disc diffusion assay. Especially, the antimicrobial activity of Psoraleae semen, which showed different activity on pathogenic Gram-positive and Gram-negative bacteria, was evaluated by MIC (minimal inhibition concentration) and biofilm formation assay. The effect of Psoraleae semen extract on bacterial cell membranes was examined by measurement of protein leakage (optical density at 280 nm) and scanning electron microscope (SEM). No clear zone was formed on discs containing Gram-negative bacteria, but Gram-positive bacteria exhibited clear zones. The MICs of Psoraleae semen extract were 8 μg/mL for Streptococcus mutans, and 16 μg/mL for Enterococci and Staphylococcus aureus. In addition, biofilm formation was inhibited at concentration 8-16 μg/mL. Protein leakage values and SEM images revealed that cell membranes of Gram-positive bacteria were impaired following exposure to the extract. Further, the extract inhibited the growth of Listeria monocytogenes in sausages. These results indicate that Psoraleae semen extract could be utilized as a natural antimicrobial agent against Gram-positive bacteria.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgements

키워드

antibacterial activity natural antimicrobials therapeutic herbal plants