흰쥐 해마절편에서 포도당/산소 고갈에 의한 5-hydroxytryptamine 유리변동에 미치는 Adenosine의 영향

• 영문명: Effect of Adenosine on the Release of [3H]-5-hydroxytryptamine during Glucose/Oxygen Deprivation from Rat Hippocampal Slices
• 발행기관: 대한생리학회-대한약리학회
• 저자명: 이경은(Kyung Eun Lee) 차광은(Kwang Eun Cha) 배영숙(Young Sook Pae)
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제6호, 657~664쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

The effects of adenosine, adenosine A₁ receptor antagonist (DPCPX), or NMDA receptor antagonist (APV) on the spontaneous release of [³H]-5-hydroxytryptamine ([³H]-5-HT) during normoxic/normoglycemic or hypoxic/hypoglycemic period were studied in the rat hippocampal slices. The hippocampus was obtained from the rat brain and sliced 400 Ռm thickness with the tissue slicer. After 30 min s preincubation in the normal buffer, the slices were incubated for 30 min in a buffer containing [³H]-5-HT (0.1 ㄍM, 74 ㄍCi/8 ml) for uptake, and washed. To measure the release of [³H]-5-HT into the buffer, the incubation medium was drained off and refilled every ten minutes through sequence of 14 tubes. Induction of glucose/oxygen deprivation (GOD; medium depleting glucose and gassed with 95% N₂/5% CO₂) was done in 6th and 7th tube. The radioactivities in each buffer and the tissue were counted using liquid scintillation counter and the results were expressed as a percentage of the total radioactivities.

When slices were exposed to GOD for 20 mins, the spontaneous release of [³H]-5-HT was markedly increased and this increase of [³H]-5-HT release was blocked by adenosine (10 ㄍM) or DL-2-amino-5-phosphonovaleric acid (APV; 30 ㄍM). Adenosine A₁ receptor specific antagonist, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) exacerbate GOD-induced increase of spontaneous release of [³H]-5-HT.

These results suggest that Adenosine may play a role in the GOD-induced spontaneous release of [³H]-5-HT through adenosine A₁ receptor activity.

키워드

Adenosine glucose/oxygen deprevation hippocampal slice [³H]-5-hydroxytryptamine release