Cost-effectiveness and other considerations for different research techniques applied in ancient DNA analysis

• 발행기관: 대한해부학회
• 저자명: Jong Ha Hong Hisashi Fujita Jaehyup Kim Dong Hoon Shin
• 간행물 정보: 『Anatomy and Cell Biology』Vol.58(1), 8~13쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2025.03.31

국문 초록

Ancient DNA (aDNA) analysis has developed rapidly since it first emerged in the 1980s, becoming an almostindispensable tool in anthropological and archaeological sciences. Earlier aDNA study was based on the polymerase chainreaction (PCR) technique, with which, unfortunately, modern DNA contamination and other authenticity issues were oftenincurred. These technical hurdles were soon overcome by application of advancements in the forms of the next generationsequencing (NGS) technique and others. However, since NGS requires money, time, and, in the case of large projects,manpower as well, genetic analysis of some ancient samples considered to be insignificant is commonly delayed or, in theworst cases, neglected entirely. We acknowledge that as a diagnostic tool in aDNA analysis, PCR is less accurate than NGSand more easily affected by modern DNA contamination; but it also has advantages, such as simplicity, time-saving, andgreater ease of interpretation, among others. The role of PCR in aDNA analysis, then, should be reconsidered.

목차

Introduction

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References

키워드

DNA ancient Commerce Efficiency Polymerase chain reaction High-throughput nucleotide sequencing