Alpha feto protein(AFP)검사 키트의 유효성 평가실험

• 영문명: Evaluation of the Kit’s Efficiency of Alpha Fetoprotein (AFP) Test
• 발행기관: 대한핵의학기술학회
• 저자명: 조현수(Hyun-su Cho) 노경운(Gyeong-woon Noh) 유태민(Tae-min You)
• 간행물 정보: 『핵의학기술』Vol.22 No.2, 101~104쪽, 전체 4쪽
• 주제분류: 의약학 > 방사선과학
• 파일형태: PDF
• 발행일자: 2018.10.30

영문 초록

Purpose Alpha fetoprotein(AFP) is a fetal serum protein that increases in germ cell tumors derived from liver cancer or egg yolk. AFP test has been used for screening of liver cancer, determination of tumor stage, determination of therapeutic effect, and fetal congenital malformations. The purpose of this study was to compare the results of the four kits, identify the advantages and disadvantages of each kit, and select the appropriate kits for our laboratory. Materials and Methods Blood samples were obtained from 89 patients attending the Seoul national university hospital. Experiments were carried out in accordance with manufacturer's instructions of four companies(A, B, C, D). The precision, recovery, linearity, and sensitivity test were performed for each kit. Results In case of the precision within the measurement, the CV value of the C kit was less than 5% at the low, middle, and high concentrations. The A, B and D kit's the CV value was less than 5% at the concentrations except the low concentration. The recovery rates of the A, B, C, and D kits were 100 ± 15%, 100 ± 30%, 100 ± 16% and 100 ± 14%, respectively. All kits showed good linearity. Sensitivity was measured as 0.5 IU/mL for A, 0.4 IU/mL for B, 0.98 IU/mL for C, and 0.3 IU/mL for D. Conclusion The CV values of the four kits were within 10%, and the correlation coefficients were close to 1 for R² = 0.978, R² = 0.992 and R² = 0.8957. As a result, they are clinically available. Therefore, each laboratory should select the appropriate kit for their experiment's environment.

목차

서론

대상 및 방법

결과

결론 및 고찰

REFERENCES

키워드

Alpha feto protein(AFP) the evaluation of kit validation