학술논문
Combination of recombinant Brucella abortus dapB and gpm proteins induces host immunity against B. abortus 544 infection using a mouse model
이용수 5
- 영문명
- Combination of recombinant Brucella abortus dapB and gpm proteins induces host immunity against B. abortus 544 infection using a mouse model
- 발행기관
- 한국예방수의학회
- 저자명
- Tran Xuan Ngoc Huy Alisha Wehdnesday Bernardo Reyes Ched Nicole Turbela Aguilar Trang Thi Nguyen Said Abdi Salad Hu Jang Lee Jin Hur Dong-Kwan Lee Suk Kim
- 간행물 정보
- 『Journal of Preventive Veterinary Medicine』Vol.47, No.4, 171~178쪽, 전체 8쪽
- 주제분류
- 의약학 > 기타의약학
- 파일형태
- 발행일자
- 2023.12.31
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국문 초록
영문 초록
The primary therapeutic approach for Brucella species infections has mainly been based on antibiotic treatment. However, the development of vaccines for brucellosis control remains controversial. Furthermore, there is currently no licensed vaccine available for human brucellosis. This study aims to evaluate the effect of a combination of recombinant protein vaccines against Brucella (B.) abortus infection using a mouse model. Two B. abortus genes, namely dapB and gpm, were cloned and expressed in competent Escherichia (E.) coli DH5α using the pCold-TF vector. Successfully cloned vectors were subjected to PCR amplification using specific primer pairs. The apparent sizes of dapB and gpm were detected at 807 bp and 621 bp, respectively. Besides, the purified recombinant proteins dapB and gpm were detected using SDS-PAGE electrophoresis with correct sizes of 82.86 kDa and 87.61 kDa, respectively. These recombinant proteins were used to immunize mice as a combined subunit vaccine (CSV) to elicit host immunity against B. abortus infection. Mice immunized with CSV exhibited increased proliferation of CD4+ and/or CD8+ T cells at week 7th and 9th before sacrifice, in comparison to the control group. Notably, CSV immunization showed a significant decrease in bacterial burden in the spleen compared to the control group. Altogether, CSV using dapB and gpm induced host adaptive immune response against Brucella infection, suggesting its potential as an effective new subunit vaccine candidate.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES
해당간행물 수록 논문
- Journal of Preventive Veterinary Medicine Vol.47, No.4 목차
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