Somatic hybridization between dendranthema grandiflorum and salpiglossis sinuata via protoplast fusion

• 발행기관: 한국육종학회
• 저자명: 이철희(Cheol Hee Lee) 백기엽(Kee Yeoup Paek) 황주광(Ju Kwang Hwang)
• 간행물 정보: 『한국육종학회지』Vol.27 No.3, 290~297쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1995.09.30

영문 초록

Somatic hybridization between axenic shoot culture of Dendranthema grandiflorum and albino cell suspension of Salpiglossis sinuata was investigated. Out of six fusion protocols and three culture media, electrofusion and KP8 medium were found to be most effective in generating somatic hybrids. Hybrid selection strategy, based on complementation for chlorophyll proficiency by the fusion of a non-dividing mesophyll protoplast system and a cytoplasmic albino mutant protoplast system was effective. Following plant regeneration, the hybridity of the regenerated plants was confirmed by morphological, cytological and biochemical analyses. Morphologically, these hybrids resembled D. grandiflorum. The somatic chromosome numbers of these plants were 2n=60. Leaf peroxidase and esterase isoenzyme analyses showed that the regenerated plants contained the banding patterns of D. grandiflorum, plus two bands associated with S. sinuata in the peroxidase profiles.

목차

ABSTRACT

Introduction

Materials and Methods

Results

Discussion

적요

Literature cited