十字花科植物의 原形質體培養과 融合에 關한 硏究 Ⅱ. 山東菜와 갓의 原形質體培養에 의한 植物體 再生

• 영문명: Studies on protoplast culture and fusion in cruciferae Ⅱ. Plant regeneration from protoplast culture of brassica campestis and brassica juncea
• 발행기관: 한국육종학회
• 저자명: Byung Jun Park(朴炳俊) Hyung Soo Suh(徐亨洙) Gun Sik Chung(鄭根植) Jae Keun Sohn(孫再根)
• 간행물 정보: 『한국육종학회지』Vol.19 No.3, 9~13쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1987.09.30

영문 초록

The experiments were conducted to determine optimum concentration of various growth regulators in fresh and regeneration medium on which affect callus formation and plant regeneration from mesophyll protoplasts of Brassica campestris CV. “Koonweesandongchae” and Brassica juncea CV. “Kyungsangot”. The formation of microcallus from protoplast could be more successfully achieved when added weekly MS liquid medium containing 1.0mg/l NAA and 0.2mg/l BA in Brassica campestris and 0.2 mg/l 2.4-D, 1.0mg/l NAA and 0.2mg/l BA in Brassica juncea. Shoot differentiation from callus, 0.3cm in diameter was accomplished in MS medium containing 0.1mg/l IAA and 2.0mg/l Zeatin in Brassica campestris and 0.1mg/l IAA and 2.0mg/l Zeatin or 0.1mg/l NAA and 1.0mg/l BA in Brassica juncea.

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