콘카나발린을 이용한 단기간 건성안 동물 모델 유용성 연구

• 영문명: The Feasibility of Short-term Dry Eye Animal Model Using Concanavalin A
• 발행기관: 대한안과학회
• 저자명: 신기철 김형찬 고정화 위원량 이진학 김미금 ,Ki Cheul Shin, M.D., Hyung Chan Kim, M.D., Jung-Hwa Ko, M.D., Won Ryang Wee, M.D.,, Jin Hak Lee, M.D.,, Mee Kum Kim, M.D.,
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume48,number5, 699~707쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.05.31

영문 초록

Purpose: To evaluate the possibility of short-term dry eye model in rabbits by injection of concanavalin A (conA) to the lacrimal and haderian gland of rabbits. Methods: We injected conA (10 mg/ml, 0.05 ml) to the lacrimal and haderian gland of rabbits twice to induce inflammation of lacrimal gland and compared with saline-injected control by lacrimal gland biopsy with H&E staining for identification of inflammation. The ratio of lacrimal secretion was evaluated by Schirmer test (preinjection vs. postinjection of conA) for 10 days and the number of goblet cells was counted in 10 consecutive high power field using impression cytology with PAS staining. The corneal & conjunctival apoptotic cell deaths were investigated with TUNEL staining 10 days after injection. Results: Infiltration of inflammatory cells and destructed normal architecture of lacrimal gland was found only in conA injected group till 10 days. The Schirmer test showed marked reduction (0.56±0.26) by 5days after injection compared with control group (1.07±0.35) (p=0.02) and its significant difference was maintained till 10days. The number of goblet cell was 9.70±5.03/×200HPF, which was statistically significant decreased compared to control (47.50±17.13/×200HPF) at 10 days (p=0.00). Apoptotic cells were increased in injected eye (26.20±4.27) compared with those in control (16.60±2.70). Conclusions: Injection of conA to lacrimal glands in rabbit shows decrease of lacrimal secretion and similar cytological changes of the cornea and conjunctiva in human dry eye patients. It suggests its possible feasibility of short-term dry eye animal model for the 10 days.

키워드

Concanavalin A, Dry eye model, Impression cytology, Rabbits, Schirmer test