춘천지역 대기부유분지의 DNA 손상효

• 영문명: Genotoxicity of Total Suspended Particulate in Chuncheon Area
• 발행기관: 대한약학회
• 저자명: 김남이(Nam Yee Kim) 임병찬(Beng-Chan Lim) 원운재(Wun-Jae Weon) 현근우(Geun-Woo Hyun) 최금종(Geum-Jong Choi) 송은정(Eun Jeong Son) 허문영(Moon Young Heo)
• 간행물 정보: 『약학회지』제51권 제6호 (2007년), 415~423쪽, 전체 9쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2007.12.31

영문 초록

This study was to evaluate the genotoxic effects of airborne particulate matters using single cell gell electrophoresis (comet assay) in A549 human lung carcinoma cells. The total suspended particulate (TSP) was collected on back-up filter in Chun cheon, Kangwon Do, South Korea from April, 2003 to February, 2005. The concentrations of TSP, B(a)P and most of heavy metals seemed to be higher in spring and winter, and lower in summer. And they showed higher concentration in the commercial areas and the residential area having more traffics than in the rural area. It was found that A549 cells interacting with the organic extract of TSP showed more DNA single-strand breaks compare to untreated cells. The genotoxicity of the organic extract of TSP was increased with the pre-treatment of S-9 mixture during the culture or with the treatment of endonuclease after cell lysis. The DNA damage by the organic extract of TSP was higher in winter and the commercial area than in summer and the rural area. This study suggests that TSP, heavy metals and B(a)P analyzed showed significant variation depend on the seasons and the areas which are correlated with the DNA damage evaluated by Comet assay, indicating that genotoxic biomarker is useful for toxicological evaluation of air quality.

키워드

totalsuspended particulate heavy metals benzo(a)pyrene DNA damage comet assay