Study of 3-Ketosteroid Dehydrogenase System Using Whole-cell-enzyme from Arthrobacter simplex

• 발행기관: 대한약학회
• 저자명: Eun Chung Park Dewey Doo-Young Ryu
• 간행물 정보: 『약학회지』제21권 제3호 (1977년), 167~171쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1977.09.30

영문 초록

A new assay method for delta-l-dehydrogenated-90-ketocorticosteroid in the presence of proteinous material or whole-cell-enzyme and 3-ketocorticosteroid has been developed. This method makes use of the linear relationship between the ratio of absorbances at 265nm and at 242nm and the fractional concentration of delta-1-3-ketosteroid. Theoretical values were calculated based on the absorbances of proteinous material at fixed concentrations of the 3-ketosteroid and delta-l-dehydrogenated-90-ketosteroid. The values obtained experimentally showed good agreement with the values theoretically predicted. The new assay method developed for the steroid mixture containing proteinous material is of some practical importance. The use of such assay method enables one to determine the enzyme activity and the rate of enzyme reaction or conversion rather quickly, easily and accurately. By the use of this assay method, the reaction kinetics of whole-cell-enzyme has also been studied. It was found that it followed the simple Michaelis-Menten type enzyme kinetics. Also the reversibility of this reaction with actively metabolizing cell was examined. It was found that delta-l-dehydrogenated-3-ketosteroid could not be hydrogenated reversibly to 3-ketosteroid by this enzyme system.