위령선 추출물의 항염활성 및 멜라닌 생성 억제효과

• 영문명: Anti-inflammatory Effect and Inhibition of Melanin Biosynthesis of Clematis mandshurica
• 발행기관: 대한약학회
• 저자명: 김예림 홍윤정 양기숙
• 간행물 정보: 『약학회지』제58권 제1호 (2014년), 47~52쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2014.02.28

영문 초록

Clematis mandshurica (Ranunculaceae) has traditionally been used as a remedy for antidiuretic, antifungal, rheumatic conditions and alleviate pain. We carried out to evaluate the anti-oxidative effect, anti-inflammatory effect and anti-melanogenic effect of ethanol extract and solvent fractions of Clematis mandshurica. The ethanol extract and the dichlo-romethane fraction of Clematis mandshurica showed an anti-oxidative effect in DPPH assay, the inhibitory activity of nitric oxide (NO) production in lipopolysaccharide (LPS) activated RAW 264.7 cell, and melanin synthesis and tyrosinase activity of B16F10 melanoma cells. They reduced NO production and melanin content in a dose-dependent manner at con-centrations of 2.5~10 µg/ml. They also suppressed iNOS and tyrosinase protein and m-RNA expressions dose dependently,assayed by western blot analysis and RT-PCR experiment.

목차

실험방법

실험결과 및 고찰

결 론

참고문헌

키워드

Clematis mandshurica NO iNOS melanin tyrosinase