Expression and localization of Rdd proteins in Xenopus embryo

• 발행기관: 대한해부학회
• 저자명: Jong Chan Lim Sayaka Kurihara Rie Tamaki Yutaka Mashima Mitsugu Maé no
• 간행물 정보: 『Anatomy and Cell Biology』Vol.47(1), 18~27쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2014.03.30

영문 초록

The previous study has shown that repeated D domain-like (Rdd) proteins, a group of novel secretory proteins consisting of repeated domains of a cysteine-rich sequence, are involved in the process of blood vessel formation in Xenopus embryo. We performed further experiments to examine the localization of Rdd proteins in embryogenesis. Detection of tagged Rdd proteins expressed in blastomeres showed that Rdd proteins formed a high molecular weight complex and existed in the extracellular space. A rabbit antibody against the Rdd synthetic peptide identified a single band of 28 kD in embryonic tissue extract. By whole-mount immunostaining analysis, signal was detected in the regions of inter-somites, vitelline veins, and branchial arches at the tailbud stage. Staining of Rdd was remarkably reduced in the embryos injected with vascular endothelial growth factor Morpholino. We suggest that Rdd proteins interact with a molecule(s) associated with vascular precursor cells.

목차

Abstract

Introduction

Materials and Methods

Results

Discussion

References

키워드

Vascular cells Frog Vascular endothelial growth factor Antibody Cysteine-rich