역상 액체 크로마토그래피에서 벤젠류의 머무름 메커니즘

• 영문명: Retention Mechanism of Benzenes in Reversed-Phase Liquid Chromatography
• 발행기관: 대전대학교 환경문제연구소
• 저자명: 이인호(In-Ho Lee) 전선주(Sun-Joo Jeon)
• 간행물 정보: 『環境問題硏究所 論文集』第4號, 91~105쪽, 전체 15쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1999.02.28

영문 초록

The purpose of this study is to investigate the retention mechanism. which is the key to the prediction of retention and to the systematical optimization for benzenes in reversed-phase liquid-solid chromatography. The retention data. capacity factors(k') of twelve benzenes was measured in the systems containing 75-100% of methanol and acetonitrile as organic modifiers. and PRP-l column. From the retention data, two extensive approaches are examined in order to elucidate the retention mechanism : 1) the comparison of the retention data of the solutes containing different substituents : 2) the relationship between the thermodynamic values and the retention data, i.e., van’t Hoff plots and enthalpy- entropy compensation phenomena. The retention behaviors of benzenes having different substituents. such as normal, iso. and tertiary alkyl groups. are well interpreted in both mobile phases by the liquid-solid adsorption phenomenon. which is closely related with displacement mechanism. The effect of temperature on retention is investigated by van' t Hoff plots in the temperature range of 35-55℃. The linearity(R>0.999) means that the retention of solutes is consistent in the above temperature range. From the observation of enthalpy-entropy compensation phenomenon. some conclusions interpreting the retention mechanism are drawn as follows : 1) the retention mechanism of solutes depends on the type of organic modifier in hydro-organic media ; 2) in methanol-water mobile phase. the retention mechanism of benzenes is consistent and identical in the range of 75-100% methanol-water ; 3) on the other hand. in the case of acetonitrile-water mobile phase, the retention mechanism depends on the acetonitrile composition. It means that the retention mechanism can not be explained only by a simple mechanism. Consequently. the retention of solutes is controlled by the displacement mechanism in methanol-water mobile phase. while the mixed mechanism. involving concurrent adsorption and partition phenomena. must be considered in acetonitrile-water mobile phase.

목차

ABSTRACT

1. 서론

2. 실험 방법

3. 결과 및 고찰

4. 결론

참고문헌