PCR 마커를 이용한 국내 밀 품종의 고분자 글루테닌 Glu-B1 대립유전자 조성 평가

• 영문명: Assessment of Allele Composition of HMW-GS Glu-B1 Locus in Domestic Wheat Cultivars Using PCR-based Markers
• 발행기관: 한국육종학회
• 저자명: 이명희(Myoung Hui Lee) 김경민(Kyeong-Min Kim) 최명구(Myoung-Goo Choi) 강천식(Chon-Sik Kang) 윤미라(Mira Yoon) 장기창(Ki-Chang Jang) 최창현(Changhyun Choi)
• 간행물 정보: 『한국육종학회지』Vol.56 No.3, 257~268쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2024.09.01

국문 초록

In common wheat (Triticum aestivum L.), the protein content and glutenin proteincomposition are the key quality-determining parameters. Allelic variations, especially inhigh-molecular-weight glutenin subunits (HMW-GSs), affect bread quality significantly. The HMW-GS Glu-1 locus consists of two tightly linked genes encoding x- and y-typesubunits that exhibit highly variable frequencies. In this study, we evaluated Glu-B1 allelesusing allele-specific PCR markers in 44 domestic wheat cultivars. The composition ofGlu-1Bx7+Glu-1By8 in the 24 cultivars was either Glu-1Bx7+Glu-1By8, Glu-1Bx7*+Glu-1By8, or Glu-1Bx7*+Glu-1Bx8*. In addition, the two cultivars initially identified Glu-1Bx7+Glu-1By8* were corrected to Glu-1Bx7*+Glu-1By8*. Seven cultivars previouslyclassified as having Glu-1Bx7+Glu-1By9 composition contained Glu-1Bx7*+ Glu-1Bx9. The allele composition of the cultivar was identified as Glu-1Bx20+Glu-1By20 instead ofGlu-1By20. The HMW-GSs of 21 wheat varieties were analyzed using ultra-performanceliquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results will be helpful for evaluating the composition of Glu-B1 alleles in domesticwheat and accurately assessing the quality of domestic wheat flour.

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키워드

domestic wheat high-molecular-weight glutenin subunit Glu-1Bx Glu-1By PCR-based marker