Segregation and linkage of allozymes in megagametophyte tissues of pinus thunbergii parl

• 발행기관: 한국육종학회
• 저자명: 최완용(Wan Yong Choi) 여진기(Jin Kie Yeo) 서강욱(Gang Uk Suh) 김종한(Jong Han Kim)
• 간행물 정보: 『한국육종학회지』Vol.26 No.4, 375~383쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1994.12.30

영문 초록

As a first step in the study of genetics of Pinus thunbergii Parl., the inheritance and the linkage of the detected polymorphisms were elucidated. Megagametophyte tissues of individual seeds from a single clone were analysed by starch gel electrophoresis. Seven enzyme systems studied comprised a minimum of 17 isozyme loci. Among them, 11 were polymophic. Segregation of isozymes in megagametophytes of heterozygous maternal clones indicated no significant departure from the expected 1 : 1 ratio in each locus. Significant linkage, as attested by the 95% confidence interval, was recognized in six pairwise combinations. The pairs of the gene loci ME-B/LAP-A and ADH-B/MNR-C were closely linked with estimated mean recombination values of 0.153 and 0.175, respectively. The remaining tested pairs (ADH-B/ LAP-B, ME-B/MDH-C, MNR-B/MDH-C, and MNR-A/MNR-B) appeared to be weakly linked. The gene loci MNR-C, ADH-B, and LAP-B could be linearly arranged in one linkage group, while the loci LAP-A, ME-B, MDH-C, MNR-A, and MNR-B seemed to belong to the other linkage group. Considerable variation with respect to the estimated recombination frequencies was observed for the same pair of loci among some clones. A comparsion with other conifer species showed that the gene orders and distances were relatively well conserved among conifer species.

목차

ABSTRACT

Introduction

Materials and Methods

Results and Discussion

적요

References

키워드

Pinus thunbergii Polymorphism In heritance Linkage Recombination